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recombinant human mmp12  (R&D Systems)


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    R&D Systems recombinant human mmp12
    Recombinant Human Mmp12, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+human+mmp+12/Recombinant+Human+MMP-12+Protein%2C+CF/pm24900526__ml3001193_si_001-121-5-8
    Average 93 stars, based on 16 article reviews
    recombinant human mmp12 - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Recombinant:

    Article Title: A new transcriptional role for matrix metalloproteinase-12 in antiviral immunity.
    Article Snippet: nature medicine advance online publication Interferons1 and macrophages are important in antiviral immunity2.. MMP-12 (ref. 3; also known as macrophage metalloelastase) is a secreted protease that degrades extracellular matrix during inflammatory tissue destruction4–6.. However, like other MMPs that have been shown to act as regulators of extracellular homeostasis and innate immunity7,8—with many new substrates and hence new functions in diverse processes9—MMP-12 is also protective, with antitumorigenic10, anti-inflammatory11 and antibacterial activities12.

    Article Title: Matrix metalloproteinase-12 (MMP-12) SNP affects MMP activity, lung macrophage infiltration and protects against emphysema in COPD.
    Article Snippet: .. Casein zymography was used to perform analysis of activity as detailed elsewhere.12 Changes to the protocol were 12% casein gels (Invitrogen, Paisley, UK) and quantification against 10 ng recombinant human MMP-12 (R&D Systems, Minneapolis, USA). .. Images were scanned and analysed using Image J V.1.40 (Wayne Rasband, NIH, USA).

    Article Title: Renal protective roles of macrophage matrix metalloproteinase-12 in mice with obstructed kidneys.
    Article Snippet: For the analysis of cytokines from BMDMs, cells were cultured with 100 ng/mL of lipopolysaccharide (LPS) (L2018; Sigma- Aldrich) for 4 h, then mRNA expressions of inflammatory cytokines were analysed using quantitative real- time reverse transcriptase polymerase chain reaction (PCR) as described below. .. To examine cytokine suppression by MMP- 12, recombinant human MMP- 12 (rhMMP- 12) (#917- MP; R&D Systems) were supplemented with 0.02 μM LPS, and BMDMs were cultured for 4 h. .. Western blot analysis was performed using previously described protocols.14 Protein lysates of cells were prepared using cell lysis buffer (Cell Signalling Technology) according to the protocols from the manufacturer.

    Article Title: MMP-12, Secreted by Pro-Inflammatory Macrophages, Targets Endoglin in Human Macrophages and Endothelial Cells
    Article Snippet: .. Recombinant human MMP-12 (R&D Systems, Minneapolis, MN, USA) activity was determined with a quenched fluorogenic peptide Mca-PLGL-Dpa-AR-NH2 (R&D Systems, Minneapolis, MN, USA), as previously described [ ]. .. Briefly, MMP-12 was activated by incubation with an assay buffer (50 mM Tris, 10 mM CaCl 2 , 150 mM NaCl, 0.05% Brij-35; pH 7.5) at 37 °C for 30 h. The fluorescence intensity was measured with a Varioskan flash spectral scanning multimode reader (Thermo Scientific, Waltham, MA, USA).

    Sequencing:

    Article Title: A new transcriptional role for matrix metalloproteinase-12 in antiviral immunity.
    Article Snippet: nature medicine advance online publication Interferons1 and macrophages are important in antiviral immunity2.. MMP-12 (ref. 3; also known as macrophage metalloelastase) is a secreted protease that degrades extracellular matrix during inflammatory tissue destruction4–6.. However, like other MMPs that have been shown to act as regulators of extracellular homeostasis and innate immunity7,8—with many new substrates and hence new functions in diverse processes9—MMP-12 is also protective, with antitumorigenic10, anti-inflammatory11 and antibacterial activities12.

    Zymography:

    Article Title: Matrix metalloproteinase-12 (MMP-12) SNP affects MMP activity, lung macrophage infiltration and protects against emphysema in COPD.
    Article Snippet: .. Casein zymography was used to perform analysis of activity as detailed elsewhere.12 Changes to the protocol were 12% casein gels (Invitrogen, Paisley, UK) and quantification against 10 ng recombinant human MMP-12 (R&D Systems, Minneapolis, USA). .. Images were scanned and analysed using Image J V.1.40 (Wayne Rasband, NIH, USA).

    Activity Assay:

    Article Title: Matrix metalloproteinase-12 (MMP-12) SNP affects MMP activity, lung macrophage infiltration and protects against emphysema in COPD.
    Article Snippet: .. Casein zymography was used to perform analysis of activity as detailed elsewhere.12 Changes to the protocol were 12% casein gels (Invitrogen, Paisley, UK) and quantification against 10 ng recombinant human MMP-12 (R&D Systems, Minneapolis, USA). .. Images were scanned and analysed using Image J V.1.40 (Wayne Rasband, NIH, USA).

    Article Title: MMP-12, Secreted by Pro-Inflammatory Macrophages, Targets Endoglin in Human Macrophages and Endothelial Cells
    Article Snippet: .. Recombinant human MMP-12 (R&D Systems, Minneapolis, MN, USA) activity was determined with a quenched fluorogenic peptide Mca-PLGL-Dpa-AR-NH2 (R&D Systems, Minneapolis, MN, USA), as previously described [ ]. .. Briefly, MMP-12 was activated by incubation with an assay buffer (50 mM Tris, 10 mM CaCl 2 , 150 mM NaCl, 0.05% Brij-35; pH 7.5) at 37 °C for 30 h. The fluorescence intensity was measured with a Varioskan flash spectral scanning multimode reader (Thermo Scientific, Waltham, MA, USA).

    Cell Culture:

    Article Title: Renal protective roles of macrophage matrix metalloproteinase-12 in mice with obstructed kidneys.
    Article Snippet: For the analysis of cytokines from BMDMs, cells were cultured with 100 ng/mL of lipopolysaccharide (LPS) (L2018; Sigma- Aldrich) for 4 h, then mRNA expressions of inflammatory cytokines were analysed using quantitative real- time reverse transcriptase polymerase chain reaction (PCR) as described below. .. To examine cytokine suppression by MMP- 12, recombinant human MMP- 12 (rhMMP- 12) (#917- MP; R&D Systems) were supplemented with 0.02 μM LPS, and BMDMs were cultured for 4 h. .. Western blot analysis was performed using previously described protocols.14 Protein lysates of cells were prepared using cell lysis buffer (Cell Signalling Technology) according to the protocols from the manufacturer.



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    Integrin β6 is a proteolysis‐resistant mediator of CRC tumour cell adhesion to endothelial cells. (A) Lysates of HT‐29 and DLD‐1 cells harvested in the presence of 0.05% trypsin or 5 mM EDTA‐PBS were collected, and the expression of integrins αv, β6, β1 and β5 was analysed using western blotting. Blots show the full‐length proteins as well as the trypsin‐induced protein fragments. GAPDH was used as a loading control. (B) Adhesion of cytopainter‐stained ITGB6 KO or NT cells harvested by trypsinization or 5 mM PBS‐EDTA to an IL‐1β‐stimulated HUVEC monolayer under static conditions. Arrow points towards an adherent cell. Scale bar: 250 μm. The quantification is shown below. Mann–Whitney U test; ns, not significant; ** p < 0.01. (C) The cancer‐related survival (60‐month cut‐off) of CRC patients who did not receive neoadjuvant therapy and had no residual tumour (R0) was explored. Based on MMP11 <t>and</t> <t>MMP12</t> RNA expression levels, patients were divided into high‐ and low‐MMP groups. ITGB6 ‐dependent survival was investigated in these groups. The number of patients per group is indicated by ‘ n ’. Cox proportional hazards survival regression. (D) Recombinant integrins αvβ6, αvβ1, and αvβ5 were incubated with MMP‐12 at an enzyme‐to‐substrate ratio of 1:10 for 1 h. Proteolytic cleavage of integrins was evaluated via western blotting.
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    Integrin β6 is a proteolysis‐resistant mediator of CRC tumour cell adhesion to endothelial cells. (A) Lysates of HT‐29 and DLD‐1 cells harvested in the presence of 0.05% trypsin or 5 mM EDTA‐PBS were collected, and the expression of integrins αv, β6, β1 and β5 was analysed using western blotting. Blots show the full‐length proteins as well as the trypsin‐induced protein fragments. GAPDH was used as a loading control. (B) Adhesion of cytopainter‐stained ITGB6 KO or NT cells harvested by trypsinization or 5 mM PBS‐EDTA to an IL‐1β‐stimulated HUVEC monolayer under static conditions. Arrow points towards an adherent cell. Scale bar: 250 μm. The quantification is shown below. Mann–Whitney U test; ns, not significant; ** p < 0.01. (C) The cancer‐related survival (60‐month cut‐off) of CRC patients who did not receive neoadjuvant therapy and had no residual tumour (R0) was explored. Based on MMP11 <t>and</t> <t>MMP12</t> RNA expression levels, patients were divided into high‐ and low‐MMP groups. ITGB6 ‐dependent survival was investigated in these groups. The number of patients per group is indicated by ‘ n ’. Cox proportional hazards survival regression. (D) Recombinant integrins αvβ6, αvβ1, and αvβ5 were incubated with MMP‐12 at an enzyme‐to‐substrate ratio of 1:10 for 1 h. Proteolytic cleavage of integrins was evaluated via western blotting.
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    Image Search Results


    Integrin β6 is a proteolysis‐resistant mediator of CRC tumour cell adhesion to endothelial cells. (A) Lysates of HT‐29 and DLD‐1 cells harvested in the presence of 0.05% trypsin or 5 mM EDTA‐PBS were collected, and the expression of integrins αv, β6, β1 and β5 was analysed using western blotting. Blots show the full‐length proteins as well as the trypsin‐induced protein fragments. GAPDH was used as a loading control. (B) Adhesion of cytopainter‐stained ITGB6 KO or NT cells harvested by trypsinization or 5 mM PBS‐EDTA to an IL‐1β‐stimulated HUVEC monolayer under static conditions. Arrow points towards an adherent cell. Scale bar: 250 μm. The quantification is shown below. Mann–Whitney U test; ns, not significant; ** p < 0.01. (C) The cancer‐related survival (60‐month cut‐off) of CRC patients who did not receive neoadjuvant therapy and had no residual tumour (R0) was explored. Based on MMP11 and MMP12 RNA expression levels, patients were divided into high‐ and low‐MMP groups. ITGB6 ‐dependent survival was investigated in these groups. The number of patients per group is indicated by ‘ n ’. Cox proportional hazards survival regression. (D) Recombinant integrins αvβ6, αvβ1, and αvβ5 were incubated with MMP‐12 at an enzyme‐to‐substrate ratio of 1:10 for 1 h. Proteolytic cleavage of integrins was evaluated via western blotting.

    Journal: International Journal of Cancer

    Article Title: Integrin β6 expression in colorectal cancer cells promotes liver metastasis through enhanced adhesion to endothelial fibronectin

    doi: 10.1002/ijc.35504

    Figure Lengend Snippet: Integrin β6 is a proteolysis‐resistant mediator of CRC tumour cell adhesion to endothelial cells. (A) Lysates of HT‐29 and DLD‐1 cells harvested in the presence of 0.05% trypsin or 5 mM EDTA‐PBS were collected, and the expression of integrins αv, β6, β1 and β5 was analysed using western blotting. Blots show the full‐length proteins as well as the trypsin‐induced protein fragments. GAPDH was used as a loading control. (B) Adhesion of cytopainter‐stained ITGB6 KO or NT cells harvested by trypsinization or 5 mM PBS‐EDTA to an IL‐1β‐stimulated HUVEC monolayer under static conditions. Arrow points towards an adherent cell. Scale bar: 250 μm. The quantification is shown below. Mann–Whitney U test; ns, not significant; ** p < 0.01. (C) The cancer‐related survival (60‐month cut‐off) of CRC patients who did not receive neoadjuvant therapy and had no residual tumour (R0) was explored. Based on MMP11 and MMP12 RNA expression levels, patients were divided into high‐ and low‐MMP groups. ITGB6 ‐dependent survival was investigated in these groups. The number of patients per group is indicated by ‘ n ’. Cox proportional hazards survival regression. (D) Recombinant integrins αvβ6, αvβ1, and αvβ5 were incubated with MMP‐12 at an enzyme‐to‐substrate ratio of 1:10 for 1 h. Proteolytic cleavage of integrins was evaluated via western blotting.

    Article Snippet: MMP‐12 (R&D Systems, Catalogue #: 917‐MPB) was activated in a concentration of 50 ng/μL by incubation in TCN buffer (10 mM Tris, 150 mM NaCl, 5 mM CaCl2, pH 7.5) at 37°C for 2 h. Following enzyme activation, the recombinant human integrins (integrin αvβ6; Catalogue #: T6‐H52E1, integrin αvβ1; Catalogue #: IT1‐H52E1, integrin αvβ5; Catalogue #: IT5‐H52W5, AcroBiosystems, Newark, DE, USA) were added in an enzyme‐to‐substrate ratio of 1:10 in TCN buffer for 1 h (MMP‐12) at 37°C.

    Techniques: Expressing, Western Blot, Control, Staining, MANN-WHITNEY, RNA Expression, Recombinant, Incubation